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Western blot analysis of extracts from MCF-7 cells lyastes.using MCJ Rabbit mAb [VRG3] at dilution of 1:1000 incubated at 4℃ over night
Protein names :DNAJC15,DNAJD1,GIG22,HSD18,DJC15_HUMAN,DnaJ homolog subfamily C member 15
UniProtID :Q9Y5T4
MASS(da) :16,383
MW(kDa) :16 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human MCJ.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :MCF-7 cells lyastes
Function : Negative regulator of the mitochondrial respiratory chain. Prevents mitochondrial hyperpolarization state and restricts mitochondrial generation of ATP (By similarity). Acts as an import component of the TIM23 translocase complex. Stimulates the ATPase activity of HSPA9..
Tissue specificity :Expressed at highest levels in heart, followed by liver and kidney..
Subcellular locationi :Mitochondrion inner membrane,Single-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.