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Western blot analysis of extracts from MCF-7 cells lyastes.using SEMA3D Rabbit mAb [28U6] at dilution of 1:1000 incubated at 4℃ over night
Protein names :SEMA3D,UNQ760/PRO1491,SEM3D_HUMAN,Semaphorin-3D
UniProtID :O95025
MASS(da) :89,651
MW(kDa) :90 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human SEMA3D.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :MCF-7 cells lyastes
Function : Induces the collapse and paralysis of neuronal growth cones. Could potentially act as repulsive cues toward specific neuronal populations. Binds to neuropilin (By similarity)..
Subcellular locationi :Secreted.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.