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MAGE-A4 Rabbit mAb [7WQR]Cat NO.: A63300

Western blot(SDS PAGE) analysis of extracts from kyse-150.Using MAGE-A4 Rabbit mAb [7WQR] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :MAGEA4,MAGE4,MAGA4_HUMAN,Melanoma-associated antigen 4

UniProtID :P43358

MASS(da) :34,899

MW(kDa) :40-50 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human MAGE-A4

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :kyse-150

Function : Regulates cell proliferation through the inhibition of cell cycle arrest at the G1 phase (PubMed:22842486). Also negatively regulates p53-mediated apoptosis (PubMed:22842486)..

Tissue specificity :Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes and placenta.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.