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Western blot(SDS PAGE) analysis of extracts from kyse-150.Using MAGE-A4 Rabbit mAb [7WQR] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :MAGEA4,MAGE4,MAGA4_HUMAN,Melanoma-associated antigen 4
UniProtID :P43358
MASS(da) :34,899
MW(kDa) :40-50 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human MAGE-A4
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :kyse-150
Function : Regulates cell proliferation through the inhibition of cell cycle arrest at the G1 phase (PubMed:22842486). Also negatively regulates p53-mediated apoptosis (PubMed:22842486)..
Tissue specificity :Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes and placenta.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.