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Western blot analysis of extracts from K-562 cells lyastes.using CKLF Rabbit mAb [F708] at dilution of 1:1000 incubated at 4℃ over night
Protein names :CKLF,CKLF1,HSPC224,UNQ410/PRO772,CKLF_HUMAN,Chemokine-like factor
UniProtID :Q9UBR5
MASS(da) :17,170
MW(kDa) :17 kda
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human CKLF.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :K-562 cells lyastes
Function : May play an important role in inflammation and regeneration of skeletal muscle (PubMed:11415443). Essential for embryonic development (By similarity).., [Isoform 1]: Has chemotactic response in monocytes, neutrophils and lymphocytes (PubMed:11415443). Binds CCR4 (PubMed:16137713)..
Tissue specificity :Isoform 1, isoform 2, isoform 3 and isoform 4 have highest expression levels in adult spleen, lung, testis, ovary, peripheral blood leukocyte, placenta, pancreas, and in fetal brain, skeletal muscle, thymus and heart. Lower expression levels in adult skeletal muscle, liver, thymus colon, prostate and fetal spleen and liver..
Subcellular locationi :[Isoform 1]: Secreted.,[Isoform 2]: Membrane,Multi-pass membrane protein.,[Isoform 4]: Membrane,Multi-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.