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Western blot analysis of extracts from 293 cells lyastes.using MGAT1 Rabbit mAb [4ZGH] at dilution of 1:1000 incubated at 4℃ over night
Protein names :MGAT1,GGNT1,GLCT1,GLYT1,MGAT,MGAT1_HUMAN,Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
UniProtID :P26572
MASS(da) :50,878
MW(kDa) :51 kda
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human MGAT1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :293 cells lyastes
Function : Initiates complex N-linked carbohydrate formation. Essential for the conversion of high-mannose to hybrid and complex N-glycans..
Subcellular locationi :Golgi apparatus membrane,Single-pass type II membrane protein. Cytoplasm, perinuclear region.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.