NIT1 Rabbit mAb [6V96]Cat NO.: A42615

Protein names ：NIT1,NIT1_HUMAN,Deaminated glutathione amidase

UniProtID ：Q86X76

MASS(da) ：35,896

MW(kDa) ：32 kda

Form ：Liquid

Purification ：Protein A purification

Host ：Rabbit

Isotype ：IgG

sensitivity ：Endogenous

Reactivity ：Human

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with a synthetic peptide of Human NIT1.

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ：293T cells lyastes

Function ： Catalyzes the hydrolysis of the amide bond in N-(4-oxoglutarate)-L-cysteinylglycine (deaminated glutathione), a metabolite repair reaction to dispose of the harmful deaminated glutathione. Plays a role in cell growth and apoptosis: loss of expression promotes cell growth, resistance to DNA damage stress and increased incidence to NMBA-induced tumors. Has tumor suppressor properties that enhances the apoptotic responsiveness in cancer cells,this effect is additive to the tumor suppressor activity of FHIT. It is also a negative regulator of primary T-cells..

Tissue specificity ：Detected in heart, brain, placenta, liver, skeletal muscle, kidney and pancreas..

Subcellular locationi ：[Isoform 2]: Mitochondrion.,[Isoform 1]: Cytoplasm.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.