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RNF185 + RNF5/NG2 Rabbit mAb [B4LT]Cat NO.: A20148

Western blot analysis of extracts from HeLa cells lyastes.using RNF185 + RNF5/NG2 Rabbit mAb [B4LT] at dilution of 1:1000 incubated at 4℃ over night

  • SizePriceIn stock
  • 100ul2088.00库存充足
  • 200ul3098.00库存充足
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Product information

Protein names :RNF185,RN185_HUMAN,E3 ubiquitin-protein ligase RNF185

UniProtID :Q96GF1

MASS(da) :20,459

MW(kDa) :20 kda

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human RNF185 + RNF5/NG2.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HeLa cells lyastes

Function : E3 ubiquitin-protein ligase that regulates selective mitochondrial autophagy by mediating 'Lys-63'-linked polyubiquitination of BNIP1 (PubMed:21931693). Acts in the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway, which targets misfolded proteins that accumulate in the endoplasmic reticulum (ER) for ubiquitination and subsequent proteasome-mediated degradation (PubMed:27485036). Protects cells from ER stress-induced apoptosis (PubMed:27485036). Responsible for the cotranslational ubiquitination and degradation of CFTR in the ERAD pathway (PubMed:24019521). Also acts as a regulator of the innate antiviral response by catalyzing 'Lys-27'-linked polyubiquitination of CGAS at 'Lys-173' and 'Lys-384', thereby promoting CGAS cyclic GMP-AMP synthase activity (PubMed:28273161). Preferentially associates with the E2 enzymes UBE2J1 and UBE2J2 (PubMed:24019521)..

Tissue specificity :Ubiquitously expressed..

Subcellular locationi :Mitochondrion outer membrane,Multi-pass membrane protein. Endoplasmic reticulum membrane,Multi-pass membrane protein.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.