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SPINK1/P12 Rabbit mAb [N10Z]Cat NO.: A86851

Western blot analysis of extracts from Human pancreas tissue lyaste.using SPINK1/P12 Rabbit mAb [N10Z] at dilution of 1:1000 incubated at 4℃ over night

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Product information

Protein names :SPINK1,PSTI,ISK1_HUMAN,Serine protease inhibitor Kazal-type 1

UniProtID :P00995

MASS(da) :8,507

MW(kDa) :9 kda

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100,
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human SPINK1/P12.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :Human pancreas tissue lyaste

Function : Serine protease inhibitor which exhibits anti-trypsin activity (PubMed:7142173). In the pancreas, protects against trypsin-catalyzed premature activation of zymogens (By similarity).., In the male reproductive tract, binds to sperm heads where it modulates sperm capacitance by inhibiting calcium uptake and nitrogen oxide (NO) production..

Subcellular locationi :Secreted.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.