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Western blot analysis of extracts from Human fetal liver tissue lyaste.using PHYHD1 Rabbit mAb [2I09] at dilution of 1:1000 incubated at 4℃ over night
Protein names :PHYHD1,PHYD1_HUMAN,Phytanoyl-CoA dioxygenase domain-containing protein 1
UniProtID :Q5SRE7
MASS(da) :32,411
MW(kDa) :32 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human PHYHD1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Human fetal liver tissue lyaste
Function : 2-oxoglutarate(2OG)-dependent dioxygenase that catalyzes the conversion of 2-oxoglutarate to succinate and CO(2) in an iron-dependent manner (PubMed:21530488). However, does not couple 2OG turnover to the hydroxylation of acyl-coenzyme A derivatives, implying that it is not directly involved in phytanoyl coenzyme-A metabolism (PubMed:21530488). Does not show detectable activity towards fatty acid CoA thioesters (PubMed:21530488).., [Isoform 2]: Isoform 2 probably lacks enzyme activity.., [Isoform 3]: Isoform 3 probably lacks enzyme activity..
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.