Nestin Mouse mAb [R8GR]Cat NO.: A25018

Protein names ：NES,Nbla00170,NEST_HUMAN,Nestin

UniProtID ：P48681

MASS(da) ：177,439

MW(kDa) ：176 kDa

Form ：Liquid

Purification ：Protein A purification

Host ：Mouse

Isotype ：IgG

sensitivity ：Endogenous

Reactivity ：Human

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000
• 免疫荧光(ICC/IF)1:100,
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with a synthetic peptide of Human Nestin.

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ：U251 cells lyastes

Function ： Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells (By similarity)..

Tissue specificity ：CNS stem cells.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.