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Western blot(SDS PAGE) analysis of extracts from Raji cells.Using Mcl-1 Rabbit mAb [3DAI] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :MCL1,BCL2L3,MCL1_HUMAN,Induced myeloid leukemia cell differentiation protein Mcl-1
UniProtID :Q07820
MASS(da) :37,337
MW(kDa) :40 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human Mcl-1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Raji cells
Function : Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis..
Subcellular locationi :Membrane,Single-pass membrane protein. Cytoplasm. Mitochondrion. Nucleus, nucleoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.