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Western blot(SDS-PAGE) analysis of extracts from U-937 cells lysate.using CFD Rabbit mAb [00V0] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CFD,DF,PFD,CFAD_HUMAN,Complement factor D
UniProtID :P00746
MASS(da) :27,033
MW(kDa) : 27KDa
Form :Rabbit IgG
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity : Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human CFD.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected : U-937 cells lysate
Function : Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway.
Subcellular locationi :Secreted.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.