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Western blot(SDS-PAGE) analysis of extracts from 293T cells lysate.using CLCN1 Rabbit mAb [CN51] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CLCN1,CLC1,CLCN1_HUMAN,Chloride channel protein 1
UniProtID :P35523
MASS(da) :108,626
MW(kDa) : 109KDa
Form :Rabbit IgG
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity : Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human CLCN1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected : 293T cells lysate
Function : Voltage-gated chloride channel (PubMed:8112288, PubMed:9122265, PubMed:12456816). Plays an important role in membrane repolarization in skeletal muscle cells after muscle contraction. The CLC channel family contains both chloride channels and proton-coupled anion transporters that exchange chloride or another anion for protons (Probable). The absence of conserved gating glutamate residues is typical for family members that function as channels (Probable)..
Tissue specificity :Predominantly expressed in skeletal muscles.
Subcellular locationi :Cell membrane,Multi-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.