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Western blot(SDS-PAGE) analysis of extracts from RAW264.7 cells lysate.using GSDMC Rabbit mAb [C9L1] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :GSDMC,MLZE,GSDMC_HUMAN,Gasdermin-C [Cleaved into: Gasdermin-C, N-terminal ,Gasdermin-C, C-terminal ]
UniProtID :Q9BYG8
MASS(da) :57,692
MW(kDa) : 62KDa
Form :Rabbit IgG
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity : Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human GSDMC.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected : RAW264.7 cells lysate
Function : [Gasdermin-C]: This form constitutes the precursor of the pore-forming protein: upon cleavage, the released N-terminal moiety (Gasdermin-C, N-terminal) binds to membranes and forms pores, triggering cell death.., [Gasdermin-C, N-terminal]: Pore-forming protein that causes membrane permeabilization and pyroptosis (PubMed:27281216). Released upon cleavage and binds to membrane inner leaflet lipids. Homooligomerizes within the membrane and forms pores of 10-15 nanometers (nm) of inner diameter, triggering pyroptosis (By similarity). The functional mechanisms and physiological proteases that cleave and activate this pore-forming protein are unknown (Probable)..
Tissue specificity :Expressed mainly in trachea and spleen (PubMed:11223543). In the esophagus, expressed in differentiating cells and probably in differentiated cells. Also detected in gastric epithelium (PubMed:19051310)..
Subcellular locationi :[Gasdermin-C]: Cytoplasm, cytosol.,[Gasdermin-C, N-terminal]: Cell membrane,Multi-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.