* 产品详情- */>
Western blot(SDS-PAGE) analysis of extracts from 293T cells lysate.using HMGCR Rabbit mAb [7ELZ] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :HMGCR,HMDH_HUMAN,3-hydroxy-3-methylglutaryl-coenzyme A reductase
UniProtID :P04035
MASS(da) :97,476
MW(kDa) :100kDa
Form :Rabbit IgG
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human HMGCR.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :293T cells lysate
Function : Catalyzes the conversion of (3S)-hydroxy-3-methylglutaryl-CoA (HMG-CoA) to mevalonic acid, the rate-limiting step in the synthesis of cholesterol and other isoprenoids, thus plays a critical role in cellular cholesterol homeostasis (PubMed:2991281, PubMed:21357570, PubMed:6995544). HMGCR is the main target of statins, a class of cholesterol-lowering drugs (PubMed:11349148, PubMed:18540668)..
Tissue specificity :[Isoform 1]: Ubiquitously expressed with the highest levels in the cerebellum, fetal brain, testis, skin and adrenal gland..,[Isoform 2]: Detected in the cerebellum, fetal brain, testis and adrenal gland..,[Isoform 3]: Low abundance except in skin, esophagus, and uterine cervix..
Subcellular locationi :Endoplasmic reticulum membrane,Multi-pass membrane protein. Peroxisome membrane,Multi-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.