* 产品详情- */>
Western blot(SDS-PAGE) analysis of extracts from Jurkat cells lysate.using IRF4 Rabbit mAb [4XQ1] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :IRF4,MUM1,IRF4_HUMAN,Interferon regulatory factor 4
UniProtID :Q15306
MASS(da) :51,772
MW(kDa) : 52KDa
Form :Rabbit IgG
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity : Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human IRF4.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected : Jurkat cells lysate
Function : Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells. Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF4 and activation of genes (By similarity)..
Tissue specificity :Lymphoid cells.
Subcellular locationi :Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.