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B-Raf (T401) Rabbit mAb [L867]Cat NO.: A87758

Western blot(SDS-PAGE) analysis of extracts from C6 cells lysate PMA/TPA-treated and serum-starvation.using B-Raf (T401) Rabbit mAb [L867] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :BRAF,BRAF1,RAFB1,BRAF_HUMAN,Serine/threonine-protein kinase B-raf

UniProtID :P15056

MASS(da) :84,437

MW(kDa) : 85KDa

Form :Rabbit IgG

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity : Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around T401 of Human B-Raf.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :C6 cells lysate PMA/TPA-treated and serum-starvation

Function : Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway (PubMed:21441910, PubMed:29433126). May play a role in the postsynaptic responses of hippocampal neurons (PubMed:1508179)..

Tissue specificity :Brain and testis.

Subcellular locationi :Nucleus. Cytoplasm. Cell membrane.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.