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Western blot(SDS-PAGE) analysis of extracts from 293T cells lysate .using CDC37 (S13) Rabbit mAb [28VN] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CDC37,CDC37A,CDC37_HUMAN,Hsp90 co-chaperone Cdc37 [Cleaved into: Hsp90 co-chaperone Cdc37, N-terminally processed]
UniProtID :Q16543
MASS(da) :44,468
MW(kDa) :50KDa
Form :Rabbit IgG
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around S13 of Human CDC37.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :293T cells lysate
Function : Co-chaperone that binds to numerous kinases and promotes their interaction with the Hsp90 complex, resulting in stabilization and promotion of their activity (PubMed:8666233). Inhibits HSP90AA1 ATPase activity (PubMed:23569206)..
Subcellular locationi :Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.