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JunD (S255) Rabbit mAb [62J6]Cat NO.: A55949

Western blot(SDS-PAGE) analysis of extracts from NIH/3T3 cells lysate Anisomycin-treated.using JunD (S255) Rabbit mAb [62J6] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :JUND,JUND_HUMAN,Transcription factor JunD

UniProtID :P17535

MASS(da) :35,174

MW(kDa) : 38KDa/42Kda

Form :Rabbit IgG

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity : Human,Mouse

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around S255 of Human JunD.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :NIH/3T3 cells lysate Anisomycin-treated

Function : Transcription factor binding AP-1 sites (PubMed:9989505). Heterodimerizes with proteins of the FOS family to form an AP-1 transcription factor complex, thereby enhancing their DNA binding activity to an AP-1 consensus sequence 3'-TGA[GC]TCA-5' and enhancing their transcriptional activity (PubMed:9989505, PubMed:28981703)..

Subcellular locationi :Nucleus.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.