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Western blot(SDS-PAGE) analysis of extracts from HeLa cells lysate.using SNX4 Rabbit mAb [374G] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :SNX4,SNX4_HUMAN,Sorting nexin-4
UniProtID :O95219
MASS(da) :51,909
MW(kDa) : 52KDa
Form :Rabbit IgG
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity : Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human SNX4.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected : HeLa cells lysate
Function : Involved in the regulation of endocytosis and in several stages of intracellular trafficking (PubMed:12668730, PubMed:17994011, PubMed:32513819, PubMed:33468622). Plays a role in recycling endocytosed transferrin receptor and prevent its degradation (PubMed:17994011). Involved in autophagosome assembly by regulating trafficking and recycling of phospholipid scramblase ATG9A (PubMed:32513819, PubMed:33468622)..
Subcellular locationi :Early endosome membrane,Peripheral membrane protein,Cytoplasmic side.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.