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Western blot(SDS PAGE) analysis of extracts from Recombinant protein lysate.Using 6*His, His-Tag Rabbit mAb [HPoL] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :6*HIS,HIS,HIS tag
MW(kDa) :40 kd
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
Reactivity :ALL
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of 6*His
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Recombinant protein lysate
Function :Identify overexpressed recombinant proteins with His tags or proteins with His tags in cells.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.