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6*His, His-Tag Rabbit mAb [HPoL]Cat NO.: A88659

Western blot(SDS PAGE) analysis of extracts from Recombinant protein lysate.Using 6*His, His-Tag Rabbit mAb [HPoL] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :6*HIS,HIS,HIS tag

MW(kDa) :40 kd

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

Reactivity :ALL

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-5000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of 6*His

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :Recombinant protein lysate

Function :Identify overexpressed recombinant proteins with His tags or proteins with His tags in cells.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.