support:13376277882 | tech@naturebios.com

beta Actin Mouse mAb [D1Hg]Cat NO.: A30209

Western blot(SDS PAGE) analysis of extracts from (1) Hela cell lysate; (2) Rat brain lysate; (3) Mouse brain lysate; (4) Chicken lung lysate; (5) Rabbit testis lysate .Using beta Actin Mouse mAb [D1Hg]at dilution of 1:1000 incubated at 4℃ over night.

  • SizePriceIn stock
  • 50ul898咨询客服
  • 100ul1198咨询客服
Order Datasheet

Product information

Protein names :ACTIN; ACTB; BETA-ACTIN; beta actin; actin beta;

UniProtID :P60709

MASS(da) :41,737

MW(kDa) :42kDa

Form :Liquid

Purification :Affinity-chromatography

Host :Mouse

Isotype : IgG

sensitivity :Endogenous

Reactivity :H M R Mk Dg Ch Pg Rabbit

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human beta Actin

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :(1) Hela cell lysate; (2) Rat brain lysate; (3) Mouse brain lysate; (4) Chicken lung lysate; (5) Rabbit testis lysate

Function : Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed:29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed:29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed:29925947)..

Subcellular locationi :Cytoplasm, cytoskeleton. Nucleus.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.