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Western blot(SDS PAGE) analysis of extracts from (1) A431 cell lysate; (2) Rat brain lysate; (3) Mouse kidney lysate; (4) Chicken lung lysate; (5) Hamster spleen lysate.Using beta Tubulin Mouse mAb [Hp39]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :Beta 4 tubulin; Beta 5 tubulin; Beta1 tubulin; Tubulin beta chain; Tubulin beta class I;
UniProtID :P07437
MASS(da) :49,671
MW(kDa) :50kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Mouse
Isotype : IgG
sensitivity :Endogenous
Reactivity :H M R Mk Dg Ch Hm Rb Sh
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human beta Tubulin
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :(1) A431 cell lysate; (2) Rat brain lysate; (3) Mouse kidney lysate; (4) Chicken lung lysate; (5) Hamster spleen lysate
Function : Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Tissue specificity :Ubiquitously expressed with highest levels in spleen, thymus and immature brain..
Subcellular locationi :Cytoplasm, cytoskeleton.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.