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Actin Rabbit mAb [30J1]Cat NO.: A62837

Western blot(SDS PAGE) analysis of extracts from (1) Hela cell lysate; (2)Human fetal kidney lysate; (3)3T3 cell lysate; (4)Mouse spleen lysate; (5)PC-12 cell lysate; (6)Rat heart lysate.Using Actin Rabbit mAb [30J1]at dilution of 1:1000 incubated at 4℃ over night.

  • SizePriceIn stock
  • 50ul898.00库存充足
  • 100ul1198.00库存充足
  • 200ul1798.00库存充足
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Product information

Protein names :a actin; ACTA; ACTA1; Actin alpha skeletal muscle; Actin; actina; actine; aktin; Alpha Actin 1; Alpha skeletal muscle Actin; ASMA;CFTD; CFTD1; CFTDM; MPFD; NEM1; NEM2; NEM3; nemaline myopathy type 3;

UniProtID :P68133

MASS(da) :42,051

MW(kDa) :42kDa

Form :Liquid

Purification :Affinity-chromatography

Host :Rabbit

Isotype : IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat Chicken

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Actin

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :(1) Hela cell lysate; (2)Human fetal kidney lysate; (3)3T3 cell lysate; (4)Mouse spleen lysate; (5)PC-12 cell lysate; (6)Rat heart lysate.

Function : Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Subcellular locationi :Cytoplasm, cytoskeleton.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.