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Western blot(SDS PAGE) analysis of extracts from (1) HeLa cell lysate; (2) HepG2 cell lysate; (3) NIH/3T3 cell lysate; (4) Mouse brain lysate; (5) C6 cell lysate.Using alpha Tubulin Rabbit mAb [9mAz]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :Alpha-tubulin 1; TUBA1; TUBA1A ; Tubulin alpha 1 chain; Tubulin alpha
UniProtID :P68366
MASS(da) :49,924
MW(kDa) :50kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human alpha Tubulin
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :(1) HeLa cell lysate; (2) HepG2 cell lysate; (3) NIH/3T3 cell lysate; (4) Mouse brain lysate; (5) C6 cell lysate.
Function : Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Subcellular locationi :Cytoplasm, cytoskeleton.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.