beta Actin Mouse mAb [K8wR]Cat NO.: A17444

Protein names ：ACTIN; ACTB; BETA-ACTIN; beta actin; actin beta;

UniProtID ：P60709

MASS(da) ：41,737

MW(kDa) ：42kDa

Form ：Liquid

Purification ：Affinity-chromatography

Host ：Mouse

Isotype ： IgG2b

sensitivity ：Endogenous

Reactivity ：Human , Mouse , Monkey , Goat , Rat , Hamster , Saccharomyces cerevisiae , Arabidopsis , Pichia pastoris , Rabbit , Zebrafish , Drosophila , Leaf of Rice , Fruit of Cucumber , Chlamydomonas Reinhardtii

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with A synthesized peptide derived from human beta Actin

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ：(1) Hela; (2)Human fetal kidney lysate; (3) 3T3 cell lysate; (4) PC-12 cell lysate; (5) COS-1 cell lysate; (6) Goat muscle lysate.

Function ： Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed:29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed:29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed:29925947)..

Subcellular locationi ：Cytoplasm, cytoskeleton. Nucleus.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.