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alpha smooth muscle Actin Rabbit mAb [mHkZ]Cat NO.: A17214

Western blot(SDS PAGE) analysis of extracts from(1)A549 cell lysate; (2)C2C12 cell lysate .Using alpha smooth muscle Actin Rabbit mAb [mHkZ]at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :Actin, aortic smooth muscle, Alpha-actin-2, Cell growth-inhibiting gene 46 protein, ACTA2, ACTSA, ACTVS, GIG46

UniProtID :P62736

MASS(da) :42,009

MW(kDa) :42kDa

Form :Liquid

Purification :Affinity-chromatography

Host :Rabbit

Isotype : IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human alpha smooth muscle Actin

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected : (1)A549 cell lysate; (2)C2C12 cell lysate

Function : Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Subcellular locationi :Cytoplasm, cytoskeleton.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.