E Cadherin Rabbit mAb [bNu5]Cat NO.: A40731

Protein names ：Arc1;Cadherin 1;CAM 120/80;CD324;CDHE;E-Cad/CTF3;ECAD;Epithelial cadherin ;LCAM;Uvomorulin;CDH1;

UniProtID ：P12830

MASS(da) ：97,456

MW(kDa) ：135kDa

Form ：Liquid

Purification ：Affinity-chromatography

Host ：Rabbit

Isotype ： IgG

sensitivity ：Endogenous

Reactivity ：Human

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000
• 免疫组化(IHC)1:100
• 免疫荧光(ICC/IF)1:100
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with A synthesized peptide derived from human E Cadherin

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ： MCF7 cell lysate.

Function ： Cadherins are calcium-dependent cell adhesion proteins (PubMed:11976333). They preferentially interact with themselves in a homophilic manner in connecting cells,cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed:11976333). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.., E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.., (Microbial infection) Serves as a receptor for Listeria monocytogenes,internalin A (InlA) binds to this protein and promotes uptake of the bacteria..

Tissue specificity ：Non-neural epithelial tissues.

Subcellular locationi ：Cell junction, adherens junction. Cell membrane,Single-pass type I membrane protein. Endosome. Golgi apparatus, trans-Golgi network.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.