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Western blot(SDS PAGE) analysis of extracts from K562 cell lysate.Using P-PKA R2 (S99) Rabbit mAb [245x]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :KAP2; PRKAR2; PKR2;
UniProtID :P13861
MASS(da) :45,518
MW(kDa) :51kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat Pig
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Phospho-PKA R2 (S99)
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :K562 cell lysate.
Function : Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.
Tissue specificity :Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
Subcellular locationi :Cytoplasm. Cell membrane.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.