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Western blot(SDS PAGE) analysis of extracts from (1) A549 cell lysate; (2) Daudi cell lysate.Using CCR9 Rabbit mAb [x338]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :G-protein coupled receptor 28; GPR-9-6; CDw199;CCR9
UniProtID :P51686
MASS(da) :42,016
MW(kDa) :45kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human CCR9
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :(1) A549 cell lysate; (2) Daudi cell lysate.
Function : Receptor for chemokine SCYA25/TECK. Subsequently transduces a signal by increasing the intracellular calcium ions level.., (Microbial infection) Alternative coreceptor with CD4 for HIV-1 infection.
Tissue specificity :Highly expressed in the thymus and low in lymph nodes and spleen..
Subcellular locationi :Cell membrane,Multi-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.