HIF-2 alpha Rabbit mAb [Zf3V]Cat NO.: A88436

Protein names ：bHLHe73;ECYT4;EPAS 1;EPAS1;HIF2A;HLF;MOP 2;PASD2

UniProtID ：Q99814

MASS(da) ：96,459

MW(kDa) ：96kDa

Form ：Liquid

Purification ：Affinity-chromatography

Host ：Rabbit

Isotype ： IgG

sensitivity ：Endogenous

Reactivity ：Human,Mouse,Rat

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000
• 免疫荧光(ICC/IF)1:100
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with A synthesized peptide derived from human HIF-2 alpha

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ：(1)MCF-7 cell lysate;(2)HeLa cell lysate.

Function ： Transcription factor involved in the induction of oxygen regulated genes. Heterodimerizes with ARNT,heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters (By similarity). Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation requires recruitment of transcriptional coactivators such as CREBBP and probably EP300. Interaction with redox regulatory protein APEX1 seems to activate CTAD (By similarity)..

Tissue specificity ：Expressed in most tissues, with highest levels in placenta, lung and heart. Selectively expressed in endothelial cells.

Subcellular locationi ：Nucleus. Nucleus speckle.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.