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Western blot(SDS PAGE) analysis of extracts from THP-1 cells.Using NLRC4 Rabbit mAb [61UG] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :NLRC4,CARD12,CLAN,CLAN1,IPAF,UNQ6189/PRO20215,NLRC4_HUMAN,NLR family CARD domain-containing protein 4
UniProtID :Q9NPP4
MASS(da) :116,159
MW(kDa) :110 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human NLRC4
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :THP-1 cells
Function : Key component of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis (PubMed:15107016). The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria (By similarity)..
Tissue specificity :Isoform 2 is expressed ubiquitously, although highly expressed in lung and spleen. Isoform 1 is highly expressed in lung, followed by leukocytes especially monocytes, lymph node, colon, brain, prostate, placenta, spleen, bone marrow and fetal liver. Isoform 4 is only detected in brain.
Subcellular locationi :Cytoplasm. Cytoplasm, cytosol. Inflammasome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.