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Western blot(SDS PAGE) analysis of extracts from (1)Jurkat cell lysate; (2) Mouse brain lysate.Using FUBP1 Rabbit mAb [T3xG]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :DNA helicase V ;FBP;FUBP;Fubp1;Fubp4;FUSE-binding protein 1;HDH V;
UniProtID :Q96AE4
MASS(da) :67,560
MW(kDa) :74kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human FUBP1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :(1)Jurkat cell lysate; (2) Mouse brain lysate.
Function : Regulates MYC expression by binding to a single-stranded far-upstream element (FUSE) upstream of the MYC promoter. May act both as activator and repressor of transcription..
Subcellular locationi :Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.