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Western blot(SDS PAGE) analysis of extracts from HeLa cell lysate.Using KDEL Rabbit mAb [rU6f]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :KDEL; Lys Asp Glu Leu;
UniProtID :P24390
MASS(da) :24,542
MW(kDa) :78KD
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human KDEL
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cell lysate.
Function : Receptor for the C-terminal sequence motif K-D-E-L that is present on endoplasmic reticulum resident proteins and that mediates their recycling from the Golgi back to the endoplasmic reticulum..
Subcellular locationi :Golgi apparatus membrane,Multi-pass membrane protein. Cytoplasmic vesicle, COPI-coated vesicle membrane,Multi-pass membrane protein. Endoplasmic reticulum membrane,Multi-pass membrane protein. Endoplasmic reticulum-Golgi intermediate compartment membrane,Multi-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.