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Western blot(SDS PAGE) analysis of extracts from Raji cell lysate.Using CD20 Rabbit mAb [087d]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :B1; S7; Bp35; CD20; MS4A2; LEU-16; MS4A1
UniProtID :P11836
MASS(da) :33,077
MW(kDa) :33kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human CD20
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Raji cell lysate.
Function : B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes (PubMed:3925015, PubMed:7684739, PubMed:12920111). Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR (PubMed:7684739, PubMed:12920111, PubMed:18474602)..
Tissue specificity :Expressed on B-cells.
Subcellular locationi :Cell membrane,Multi-pass membrane protein. Cell membrane,Lipid-anchor.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.