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Western blot(SDS PAGE) analysis of extracts from (1) A431 cell lysate; (2) MCF-7 cell lysate.Using Actin (Alpha-Actin) Rabbit mAb [nZgv]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ACTA, ACTA1, ACTS, Actin, alpha skeletal muscle, Alpha-actin 1
UniProtID :P68133
MASS(da) :42,051
MW(kDa) :42kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Actin (Alpha-Actin)
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :(1) A431 cell lysate; (2) MCF-7 cell lysate.
Function : Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Subcellular locationi :Cytoplasm, cytoskeleton.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.