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Western blot(SDS PAGE) analysis of extracts from HeLa cell lysate. .Using Sodium Potassium ATPase Rabbit mAb [ehwO]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :A1A1; AT1A1; ATP1A1; Na+/K+ transporting; alpha 1 polypeptide; Na+/K+ ATPase 1; Na,K-ATPase 1; Sodium pump 1;
UniProtID :P05023
MASS(da) :112,896
MW(kDa) :100kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Sodium Potassium ATPase
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cell lysate.
Function : This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients..
Subcellular locationi :Basolateral cell membrane,Multi-pass membrane protein. Cell membrane, sarcolemma,Multi-pass membrane protein. Cell projection, axon. Melanosome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.