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Western blot(SDS PAGE) analysis of extracts from expression HeLa cell lysate.Using Talin 2 Rabbit mAb [Mi67]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :DKFZp451B1011;ILWEQ;Talin-2;talin2;TLN 2;TLN2
UniProtID :Q9Y4G6
MASS(da) :271,613
MW(kDa) :270kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Talin 2
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :expression HeLa cell lysate.
Function : As a major component of focal adhesion plaques that links integrin to the actin cytoskeleton, may play an important role in cell adhesion. Recruits PIP5K1C to focal adhesion plaques and strongly activates its kinase activity (By similarity)..
Subcellular locationi :Cytoplasm. Cell junction, focal adhesion. Cell junction, synapse. Cell membrane,Peripheral membrane protein,Cytoplasmic side. Cytoplasm, cytoskeleton.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.