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Western blot(SDS PAGE) analysis of extracts from expression NIH/3T3 cell lysate.Using CD34 Rabbit mAb [XMN5]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :Hematopoietic progenitor cell antigen CD34; CD34
UniProtID :P28906
MASS(da) :40,716
MW(kDa) :120kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat Dog
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human CD34
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :expression NIH/3T3 cell lysate.
Function : Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
Tissue specificity :Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
Subcellular locationi :Membrane,Single-pass type I membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.