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Western blot(SDS PAGE) analysis of extracts from Jurkat cell lysates treated with pervanadate .Using P-alpha Tubulin (Y272) Rabbit mAb [1VMw]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :Alpha-tubulin 3; B-ALPHA-1; FLJ25113; hum-a-tub1; hum-a-tub2; LIS3; TBA1A; TUBA1A; TUBA3; Tubulin alpha-1A chain; Tubulin alpha-3 chain; Tubulin B-alpha-1; tubulin, alpha 1a; tubulin, alpha 3; tubulin, alpha, brain-specific
UniProtID :P68363
MASS(da) :50,152
MW(kDa) :54kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Phospho-alpha Tubulin (Y272)
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Jurkat cell lysates treated with pervanadate
Function : Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Subcellular locationi :Cytoplasm, cytoskeleton.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.