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Western blot(SDS PAGE) analysis of extracts from MCF-7 cells.Using Notch3 Rabbit mAb [5J0A] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :NOTCH3,NOTC3_HUMAN,Neurogenic locus notch homolog protein 3 [Cleaved into: Notch 3 extracellular truncation,Notch 3 intracellular domain]
UniProtID :Q9UM47
MASS(da) :243,631
MW(kDa) :90, 270 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human Notch3
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :MCF-7 cells
Function : Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination (PubMed:15350543). Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity)..
Tissue specificity :Ubiquitously expressed in fetal and adult tissues.
Subcellular locationi :Cell membrane,Single-pass type I membrane protein.,[Notch 3 intracellular domain]: Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.