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Western blot(SDS PAGE) analysis of extracts from (1) Caco2 cell lysate; (2) Mouse spleen lysate.Using HAPLN1 Rabbit mAb [DIfF]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :Hyaluronan and proteoglycan link protein 1; Cartilage-linking protein 1; Cartilage-link protein; Proteoglycan link protein; HAPLN1; CRTL1
UniProtID :P10915
MASS(da) :40,166
MW(kDa) :48kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human HAPLN1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :(1) Caco2 cell lysate; (2) Mouse spleen lysate.
Function : Stabilizes the aggregates of proteoglycan monomers with hyaluronic acid in the extracellular cartilage matrix.
Tissue specificity :Widely expressed. Weakly expressed in the brain..
Subcellular locationi :Secreted, extracellular space, extracellular matrix.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.