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Western blot(SDS PAGE) analysis of extracts from (1) HeLa cell lysate; (2) K562 cell lysate.Using Flotillin 1 Rabbit mAb [a25C]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :Flotillin1 ; Flotillin 1; Reggie 2; FLOT1; Integral membrane component of caveolae;
UniProtID :O75955
MASS(da) :47,355
MW(kDa) :48kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Flotillin 1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :(1) HeLa cell lysate; (2) K562 cell lysate.
Function : May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.
Subcellular locationi :Cell membrane,Peripheral membrane protein. Endosome. Membrane, caveola,Peripheral membrane protein. Melanosome. Membrane raft.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.