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Flotillin 1 Rabbit mAb [a25C]Cat NO.: A62940

Western blot(SDS PAGE) analysis of extracts from (1) HeLa cell lysate; (2) K562 cell lysate.Using Flotillin 1 Rabbit mAb [a25C]at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :Flotillin1 ; Flotillin 1; Reggie 2; FLOT1; Integral membrane component of caveolae;

UniProtID :O75955

MASS(da) :47,355

MW(kDa) :48kDa

Form :Liquid

Purification :Affinity-chromatography

Host :Rabbit

Isotype : IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Flotillin 1

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :(1) HeLa cell lysate; (2) K562 cell lysate.

Function : May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.

Subcellular locationi :Cell membrane,Peripheral membrane protein. Endosome. Membrane, caveola,Peripheral membrane protein. Melanosome. Membrane raft.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.