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Western blot(SDS PAGE) analysis of extracts from KARPAS-299 cells.Using Noxa Rabbit mAb [992M] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :PMAIP1,NOXA,APR_HUMAN,Phorbol-12-myristate-13-acetate-induced protein 1
UniProtID :Q13794
MASS(da) :6,030
MW(kDa) :10 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human Noxa
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :KARPAS-299 cells
Function : Promotes activation of caspases and apoptosis. Promotes mitochondrial membrane changes and efflux of apoptogenic proteins from the mitochondria. Contributes to p53/TP53-dependent apoptosis after radiation exposure. Promotes proteasomal degradation of MCL1. Competes with BAK1 for binding to MCL1 and can displace BAK1 from its binding site on MCL1 (By similarity). Competes with BIM/BCL2L11 for binding to MCL1 and can displace BIM/BCL2L11 from its binding site on MCL1..
Tissue specificity :Highly expressed in adult T-cell leukemia cell line.
Subcellular locationi :Mitochondrion.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.