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Western blot(SDS PAGE) analysis of extracts from HeLa cell lysate.Using Rad21 Rabbit mAb [uPGm]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :hHR21; Nuclear matrix protein 1; NXP-1; SCC1 homolog; HR21;KIAA0078; NXP1;Rad21;
UniProtID :O60216
MASS(da) :71,690
MW(kDa) :72,128kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Rad21
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cell lysate.
Function : [Double-strand-break repair protein rad21 homolog]: As a member of the cohesin complex, involved in sister chromatid cohesion from the time of DNA replication in S phase to their segregation in mitosis, a function that is essential for proper chromosome segregation, post-replicative DNA repair, and the prevention of inappropriate recombination between repetitive regions (PubMed:11509732). The cohesin complex may also play a role in spindle pole assembly during mitosis (PubMed:11590136). In interphase, cohesins may function in the control of gene expression by binding to numerous sites within the genome (By similarity). May control RUNX1 gene expression (Probable). Binds to and represses APOB gene promoter (PubMed:25575569). May play a role in embryonic gut development, possibly through the regulation of enteric neuron development (By similarity).., [64-kDa C-terminal product]: May promote apoptosis..
Tissue specificity :Expressed in the gut (at protein level)..
Subcellular locationi :[Double-strand-break repair protein rad21 homolog]: Nucleus. Nucleus matrix. Chromosome. Chromosome, centromere. Cytoplasm, cytoskeleton, spindle pole.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.