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Western blot(SDS PAGE) analysis of extracts from (1)Hela cell lysate;(2)Human heart lysate.Using Lactate Dehydrogenase Rabbit mAb [F99s]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :LDHA; LDH-M; LDH1; PIG19; GSD11; TRG5; Proliferation inducing gene 19;
UniProtID :P00338
MASS(da) :36,689
MW(kDa) :37kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Lactate Dehydrogenase
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :(1)Hela cell lysate;(2)Human heart lysate.
Function : Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD(+)..
Tissue specificity :Predominantly expressed in anaerobic tissues such as skeletal muscle and liver..
Subcellular locationi :Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.