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Western blot(SDS PAGE) analysis of extracts from (1)Neuro-2a cell lysates; (2)K562 cell lysate.Using ELK1 Rabbit mAb [6zy3]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ELK1; ETS-domain protein ELK-1; ETS-domain protein Elk-1;
UniProtID :P19419
MASS(da) :44,888
MW(kDa) :62kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human ELK1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :(1)Neuro-2a cell lysates; (2)K562 cell lysate.
Function : Transcription factor that binds to purine-rich DNA sequences. Forms a ternary complex with SRF and the ETS and SRF motifs of the serum response element (SRE) on the promoter region of immediate early genes such as FOS and IER2. Induces target gene transcription upon JNK-signaling pathway stimulation (By similarity)..
Tissue specificity :Lung and testis.
Subcellular locationi :Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.