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Western blot(SDS PAGE) analysis of extracts from (1)HaCaT cell lysates; (2)HepG2 cell lysate.Using Cyclin B1 Rabbit mAb [Em00]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CCN-2; CCNB; CCNB1;, CCNB1-RS1; CYCB; CYCB1; G2/mitotic-specific cyclin B1;
UniProtID :P14635
MASS(da) :48,337
MW(kDa) :60kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Cyclin B1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :(1)HaCaT cell lysates; (2)HepG2 cell lysate.
Function : Essential for the control of the cell cycle at the G2/M (mitosis) transition..
Subcellular locationi :Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.