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Cyclin B1 Rabbit mAb [Em00]Cat NO.: A94146

Western blot(SDS PAGE) analysis of extracts from (1)HaCaT cell lysates; (2)HepG2 cell lysate.Using Cyclin B1 Rabbit mAb [Em00]at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :CCN-2; CCNB; CCNB1;, CCNB1-RS1; CYCB; CYCB1; G2/mitotic-specific cyclin B1;

UniProtID :P14635

MASS(da) :48,337

MW(kDa) :60kDa

Form :Liquid

Purification :Affinity-chromatography

Host :Rabbit

Isotype : IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Cyclin B1

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :(1)HaCaT cell lysates; (2)HepG2 cell lysate.

Function : Essential for the control of the cell cycle at the G2/M (mitosis) transition..

Subcellular locationi :Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.