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Western blot(SDS PAGE) analysis of extracts from HaCaT cell lysate.Using Cytokeratin 8 Rabbit mAb [el1R]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CARD2; CK-8; CK8; CYK8; cytokeratin 8; Cytokeratin-8; K2C8; K8; keratin 8; Keratin, type II cytoskeletal 8; Keratin-8; KO; KRT8; Type-II keratin Kb8;
UniProtID :P05787
MASS(da) :53,704
MW(kDa) :53kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human Mouse
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Cytokeratin 8
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HaCaT cell lysate.
Function : Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle..
Tissue specificity :Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity..
Subcellular locationi :Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.