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Western blot(SDS PAGE) analysis of extracts from (1) Firefly Luciferase transfected 293T cell lysate; (2) HeLa cell lysate; (3) NIH/3T3 cell lysate; (4) C6 cell lysate.Using Firefly Luciferase Rabbit mAb [QZ99]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :Luciferase; Firefly; Luciferin 4 monooxygenase;
UniProtID :P08659
MW(kDa) :55kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Firefly
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from Firefly Luciferase
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :(1) Firefly Luciferase transfected 293T cell lysate; (2) HeLa cell lysate; (3) NIH/3T3 cell lysate; (4) C6 cell lysate.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.